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semidry electrical transfer device  (Bio-Rad)


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    Bio-Rad semidry electrical transfer device
    Semidry Electrical Transfer Device, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/semidry+electrical+transfer+device/Trans-Blot+Turbo+Transfer+System/pmc06879876-133-15-15
    Average 90 stars, based on 1 article reviews
    semidry electrical transfer device - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Staining:

    Article Title: Dysferlin deficiency blunts β-adrenergic-dependent lusitropic function of mouse heart
    Article Snippet: .. Protein bands resolved in the gel were stained with Coomassie Blue R250 or blotted on a nitrocellulose membrane using a semidry electrical transfer device (Bio-Rad, Hercules, CA, USA). .. The nitrocellulose membranes were blocked with 1% BSA in Tris-buffered saline (TBS) and incubated with anti-dysferlin monoclonal antibody (mAb) Ham1/7B6 (Abcam Inc., Cambridge, MA, USA), anti-cardiac troponin T (TnT) mAb CT3 (Chong & Jin, 2009), anti-troponin I (TnI) mAb TnI-1 (Jin et al. 2001), an anti-ventricular myosin light chain 2 (MLC2v) rabbit mAb (Abcam), an anti-Ser282 phosphorylated cardiac myosin binding protein-C (cMyBP-C) antibody (Enzo Lifesciences, New York, USA), an anti-calmodulin kinase II (CaMKII) rabbit mAb (Abcam), or an anti-Thr286 phosphorylated CaMKII rabbit antiserum (Abcam), diluted in TBS containing 0.1% BSA, at 4°C overnight.

    Membrane:

    Article Title: Dysferlin deficiency blunts β-adrenergic-dependent lusitropic function of mouse heart
    Article Snippet: .. Protein bands resolved in the gel were stained with Coomassie Blue R250 or blotted on a nitrocellulose membrane using a semidry electrical transfer device (Bio-Rad, Hercules, CA, USA). .. The nitrocellulose membranes were blocked with 1% BSA in Tris-buffered saline (TBS) and incubated with anti-dysferlin monoclonal antibody (mAb) Ham1/7B6 (Abcam Inc., Cambridge, MA, USA), anti-cardiac troponin T (TnT) mAb CT3 (Chong & Jin, 2009), anti-troponin I (TnI) mAb TnI-1 (Jin et al. 2001), an anti-ventricular myosin light chain 2 (MLC2v) rabbit mAb (Abcam), an anti-Ser282 phosphorylated cardiac myosin binding protein-C (cMyBP-C) antibody (Enzo Lifesciences, New York, USA), an anti-calmodulin kinase II (CaMKII) rabbit mAb (Abcam), or an anti-Thr286 phosphorylated CaMKII rabbit antiserum (Abcam), diluted in TBS containing 0.1% BSA, at 4°C overnight.



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